Download Advances in Tracer Methodology: Volume 1 by F. Marott Sinex (auth.), Seymour Rothchild (eds.) PDF

By F. Marott Sinex (auth.), Seymour Rothchild (eds.)

The 5 Symposia on Advances in Tracer technique have been held every year from 1957 to 1961. The symposia have been directed to scientists who're energetic in using tracer recommendations to assist resolve their medical difficulties. The structure, a casual one-day assembly together with approximately ten papers and shutting with a cocktail hour, fostered an lively trade of data between audio system and viewers. even if the 1st symposia have been limited to using tritium as a tracer isotope, the bigger goal of the conferences used to be to disseminate info with regards to the complete isotopic tracer box. The sponsoring organisations, all actively engaged in promoting items within the nuclear box, tried to supply a noncom­ mercialized discussion board which might facilitate this trade of in­ formation. the gathering of papers offered herein represents many of the talks offered on the first symposia plus numerous acceptable papers that have seemed both in Atomlight, the bulletin of the hot England Nuclear Corp., or that have been submitted at once for inclusion during this assortment. even if all of the authors used to be given the chance to revise his paper, it truly is most likely that the various ideas or instrumentation defined may perhaps have already got been outdated through fresh improvements.

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And Dorfman, A. J. BioI. Chem. 228, 547 (1957). (4) Wilzbach, K. E. J. Am, Chem. Soc. 79, 1013 (1957). , Vaughn, M.. Anfinsen, C. B.. and Gorry, J. Science 126, 447 (1957). (6) Markovitz, A.. , and Dorfman, A. J. BioI. Chem. 234, 2343 (1959). G. and Le1oir, L. F. Biochem. J. 51,426 (1952). J. Recent Progr. in Hormone Research 12,134 (1956). (9) Wilson, A. T. Nature 182, 524 (1958); Biochim. et Biophys. Acta 40, 522 (1960). (10) Linker, A.. , and Hoffman, P. J. BioI. Chem. 219, 13 (1956). CORRELATION OF EFFICIENCY OF LABELING WITH CHEMICAL CONSTITUTION* Seymour Rothchild New England Nuclear Corp.

1% 2% Fatty Acids Combined As Esters Or Triglycerides (by GLC of methylated (12) linseed oils) 5% 5% Palmitic ......... , ...... ..... Stearic. . . . . . . . . . . . . . . . . 2% 2% Oleic.. .. ........ ....... ... 21% 21% Linoleic. . . . . . . . . . .......... 13% 12% Linolenic. . . . . . . . . . . . . . . . 49% 50% (the remaining 10% is glycerine plus traces of other compounds) Tritium Labeling by Other Methods 54 Table II. cjg Decanted Linseed Oil .................................

63, 799 (1959). (13) Jackson et al. Nucleonics 18, 8, 102 (1960). (14) Wilzbach. A. Int. Conf. Uses of Radioisotopes, Copenhagen, 1960. (15) Mottlau. J. Phys. Chem. 64, 931 (1960). SOME EXPERIENCES WITH LABELING SUGARS BY TRITIUM GAS EXPOSURE * Robert K. Crane ,t George R. Drysdale, and Kenneth H. Hawkinst Department of Biological Chemistry Washington University Medical School St. Louis, Missouri Our interest in the Wilzbach direct tritium-labeling procedure [1] was aroused by the possibilities it offered to obtain radioactive counterparts of sugars and sugar analogs for which chemical synthesis from C 14 -labeled precursors is either very difficult, prohibitively expensive, or both.

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